Application

Western blot: 1-10 μg/mL (Chemiluminescence technique). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. Antibody detects ~55kDa band in HT222 cells and mouse hippocampal membranes. {http://www.jneurosci.org/cgi/reprint/21/16/6069.pdf, Ishige, K et.al.(2001) J. Neuroscience 21(16):6069-6076.

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide/well)

Not tested in immunohistochemical methods.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Anti-Dopamine D4 Receptor Antibody, extracellular domain is an antibody against Dopamine D4 Receptor for use in ELISA & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: extracellular domain

A 10 amino acid peptide sequence from the mouse D4 receptor within the 3rd extracellular domain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1789P

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Specificity

Specific for Dopamine Receptor D4. Mouse Dopamine receptor D4 is a 387 aa, G-protein coupled and transmembrane receptor protein (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D3 or D5).

SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 90% with rat and human D4 receptors (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994; O′Malley et al. 1992). Antibody cross-reactivity with D4R from various species is not known.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.